山羊源K57荚膜型肺炎克雷伯菌毒力基因检测及遗传进化分析

doi:10.16431/j.cnki.1671-7236.2025.10.032

Abstract

Abstract: 【Objective】 The purpose of the experiment was to investigate the virulence gene profile of the goat-derived Klebsiella pneumoniae strain KPHN001 with the K57 capsule serotype,and examine the distribution of virulence genes and sequence types (STs) among K57 capsule serotype Klebsiella pneumoniae strains from various sources,in order to assess their genetic diversity and potential evolutionary features.【Method】 After resuscitation and cultivation,the virulence phenotype of KPHN001 strain was determined by string test.The serotype and virulence genes of the strain were examined by PCR method.According to multilocus sequence typing (MLST) and phylogenetic tree reconstruction of single copy homologous genes,KPHN001 strain was compared with 30 strains of K57 capsule serotype Klebsiella pneumoniae from different sources in NCBI database.【Result】 String test result showed that KPHN001 strain was unable to produce a mucoviscous string ≥5 mm in length.PCR results showed that KPHN001 strain was K57 capsule serotype,containing several virulence genes including khe,fimH,uge,kfuBC,wabG,ureA,entB,mrkD,repA and sopB,while none of the important genetic marker genes for high-virulence Klebsiella pneumoniae (hvKP),such as rmpA2,iucA and iroB,were detected.Based on the negative string test and the absence of hvKP marker genes,the KPHN001 strain was identified as a classical Klebsiella pneumoniae (cKP) strain.The MLST results showed that the ST type distribution of K57 capsule serotype Klebsiella pneumoniae strains from bovine,goat and environment exhibited diversity,while the strains from human mainly concentrated on ST412 and ST218.KPHN001 strain was classified as ST528.The combined analysis of phylogenetic tree with virulence gene heatmap indicated that strains from human exhibited significant differences in uge gene expression compared to strains from other sources,and strains from human formed independent branches,indicating that these strains might have accumulated unique genetic variation during evolution.【Conclusion】 This study elucidated the virulence gene profile of a goat-derived K57 capsule serotype Klebsiella pneumoniae strain and highlighted the genetic diversity of K57 capsule serotype strains,providing a theoretical basis for understanding their virulence mechanisms and the diagnosis and prevention of Klebsiella pneumoniae infection.

Key words: Klebsiella pneumoniae; K57 capsule serotype; virulence genes; MLST; genetic evolution

CLC Number:

S852.61

HE Meirong, LIAO Lianjie, HE Qiuyi, PAN Haoju, YANG Yingxue, CHEN Taoyu, LI Haoyang, JIANG Junming, CHEN Qiaoling, GAO Hongyan, CHEN Si, DU Li, WANG Fengyang, MAN Churiga. Virulence Gene Detection and Genetic Evolution Analysis of Goat-derived K57 Capsule Serotypes Klebsiella pneumoniae[J]. China Animal Husbandry & Veterinary Medicine, 2025, 52(10): 4874-4883.

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Virulence Gene Detection and Genetic Evolution Analysis of Goat-derived K57 Capsule Serotypes Klebsiella pneumoniae

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