壳聚糖对童鸽血清生化指标、免疫功能及肠道微生物的影响

doi:10.16431/j.cnki.1671-7236.2025.10.010

Abstract

Abstract: 【Objective】 The aim of this study was to explore the effects of adding chitosan to drinking water on the serum biochemical indicators,immune function and intestinal microbiota of squeakers in White King pigeons,and evaluate the application effect of chitosan in pigeons breeding，so as to provide theoretical basis for its development and application as an additive for pigeon feed.【Method】 Sixty 2-month-old healthy squeakers were selected and randomly divided into two groups:Control group and chitosan group.Each group was further divided into 5 replicates,with 6 pigeons in each replicate.Squeakers in control group were fed with the basal diet and normal drinking water without chitosan addition.Squeakers in chitosan group were fed with the basal diet and chitosan was added to the drinking water at a concentration of 0.2%.The experiment lasted for 42 days.Before and after the experiment,the squeakers were fasted and weighed.After the experiment,five squeakers were selected from each group to measure the changes in serum biochemical indicators,immune function,slaughter performance,meat quality,and intestinal microflora.【Result】 Compared with control group,the contents of immunoglobulin A (IgA) and IgG in serum,and gizzard index of squeakers in chitosan group were extremely significantly or significantly increased (P＜0.01 or P＜0.05).However,no significant differences were observed in total bilirubin (T-BIL),bile acids (TBA),lactate dehydrogenase (LDH),complement 3 (C3),C4 levels,thymus index,and spleen index (P＞0.05).The initial body weight,final body weight,slaughter rate,half-eviscerated yield,full-eviscerated rate,and breast muscle yield remained unaffected (P＞0.05).The pH,redness (a^*),yellowness (b^*),lightness (L^*),and drip loss rate of breast muscle of squeakers in chitosan group showed no significant changes (P＞0.05).16S rRNA gene sequencing results revealed that chitosan could increase the intestinal microbial diversity in squeakers，and significantly reduced the relative abundance of harmful bacteria such as Campylobacter (P＜0.05),and affected the intestinal adaptive response by regulating the composition of the microbiota.【Conclusion】 Under the conditions of this experiment,the addition of 0.2% chitosan to drinking water enhanced the immune function of squeaker and optimized the intestinal microecological environment,thereby benefiting their gut health.

Key words: chitosan; squeakers; biochemical indicators; immune performance; ileal microbiota

CLC Number:

S816.71

GAO Yuxin, SUN Hong, ZHANG Ran, LI Xinyi, LI Qin, YUAN Yifei, MA Hui, LIU Qing. Effects of Chitosan on Serum Biochemical Indicators,Immune Function,and Intestinal Microbiota of Squeakers[J]. China Animal Husbandry & Veterinary Medicine, 2025, 52(10): 4641-4651.

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Effects of Chitosan on Serum Biochemical Indicators,Immune Function,and Intestinal Microbiota of Squeakers

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