基于转录组和蛋白质组分析筛选绒山羊绒毛脱落相关候选基因

doi:10.16431/j.cnki.1671-7236.2025.08.001

Abstract

Abstract: 【Objective】 This study integrated transcriptomic and proteomic profiling to identify candidate genes associated with cashmere fiber shedding in cashmere goats,elucidating genetic regulatory mechanisms and establishing a theoretical framework for molecular marker-assisted breeding. 【Method】 Six 2-year-old female half-sib Hanshan White cashmere goats were randomly divided into control and melatonin (MT)-implanted groups,with three biological replicates per group.Skin tissues were collected during natural shedding in May (D5),MT-induced premature shedding in April (M4),and secondary shedding in August (M8).RNA and proteins were extracted for transcriptomic sequencing (RNA-Seq) and Label-free DIA-based proteomic quantification to identify differentially expressed genes (DEGs) and proteins (DEPs),followed by functional enrichment analysis and protein-protein interaction (PPI) network construction. 【Result】 In M4 vs D5 comparison group,332 DEGs and 47 DEPs were identified,including overlapping genes P4HA2 and DLX3,with DEGs and DEPs significantly enriched in pathways related to skin development,hair follicle cycling,PPAR signaling pathway,and PI3K-Akt signaling pathway,leading to the identification of nine key genes such as DLX3,FOXN1,and COL1A1.In M8 vs D5 comparison group,345 DEGs and 120 DEPs were identified,with overlapping genes CNDP1,KRTAP11-1,and OAT,enriched in skin development,hair follicle development,hair cycle,PPAR,and calcium signaling pathway,highlighting six key genes including KRTAP11-1,OAT,FOXN1,etc.The M8 vs M4 comparison group showed 193 DEGs and 51 DEPs primarily involved in cell projection regulation,PI3K-Akt signaling,focal adhesion,etc.with three key genes (COL1A1,COL1A2,and S100A4) identified.Among them,cysteine and methionine metabolic pathways in shedding groups were continuously activated,while the COL1A1 and FOXN1 genes repeatedly appeared in multiple comparison groups,suggesting that they might be involved as core regulatory factors in abnormal hair follicle cycles. 【Conclusion】 This study revealed distinct genetic regulatory patterns during MT-induced cashmere shedding in April and August.In the April shedding group,two overlapping genes (P4HA2 and DLX3) and two unique genes (KRT32 and DKK1) were identified,while the August secondary shedding group exhibited three overlapping genes (KRTAP11-1,OAT,and CNDP1) and one unique gene (S100A4).Persistent activation of cysteine and methionine metabolism pathways was observed across shedding groups.The results provided critical insights into the molecular mechanisms governing cashmere shedding characteristics in cashmere goats,establishing a theoretical foundation for precisely regulating hair follicle cycle and optimizing MT-induced shedding techniques alongside cashmere elongation strategies.

Key words: Hanshan White cashmere goat; transcriptome; proteome; melatonin; candidate genes

CLC Number:

S813.3

TIAN Jindong, TA La, LI Weiwei, XU Huijuan, WU Jianghong, WUDUBALA, HU Sile, CAO Wenya, SONG Hongli, FU Shaoyin, LI Chun. Screening of Candidate Genes Related to Cashmere Shedding in Cashmere Goats Based on Transcriptome and Proteome Analysis[J]. China Animal Husbandry & Veterinary Medicine, 2025, 52(8): 3497-3514.

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Screening of Candidate Genes Related to Cashmere Shedding in Cashmere Goats Based on Transcriptome and Proteome Analysis

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