Abstract: 【Objective】 The aim of this study was to investigate the effects of different dietary lipid levels on growth,body composition,liver biochemical parameters,digestive capacity,and related antioxidant indices in hybrid snakehead (Channa argus♂ × Channa maculate♀) larvae,and determine the optimal dietary lipid level for hybrid snakehead larvae. 【Method】 825 experimental fish with an initial body weight of (299.87±0.15) mg were randomly divided into 5 groups,each with 3 replicates and 55 fish per replicate.They were fed 5 different diets with lipid levels of 11.22%,13.22%,16.25%,19.28%,and 22.54%,respectively,and designated as L10,L13,L16,L19,and L22 groups.The fish were reared in net cages (0.50 m×0.30 m×0.50 m) for 28 days.At the end of the experiment,the weight and number of experimental fish in each cage were counted,and growth performance was calculated.Fifteen fish were randomly selected from each cage for body composition analysis.The entire intestine and liver were collected for the determination of biochemical parameters,digestive enzyme activity,and antioxidant enzyme activity. 【Result】 ①The fish in L19 group exhibited significantly higher final body weight,weight gain rate,and specific growth rate than L10 and L22 groups (P＜0.05),and their feed conversion ratio was significantly lower than L10,L13,and L22 groups (P＜0.05).The protein efficiency ratio of fish in L19 and L16 groups were significantly higher than that of L10 and L13 groups (P＜0.05).There were no significant differences in survival rate,condition factor,and viscerosomatic index among all groups(P＞0.05).②Experimental fish in L19 and L22 groups had significantly higher whole-body crude lipid content compared to L10,L13,and L16 groups (P＜0.05),and there were no significant differences in whole-body moisture,crude protein,and crude ash contents among all groups (P＞0.05).③In the livers of the experimental fish,the triglyceride content in L19 and L22 groups was significantly higher than L10,L13,and L16 groups (P＜0.05),the total cholesterol content in L19 group was significantly higher than L10 group (P＜0.05),and the activities of alanine transaminase and aspartate transaminase had no significant differences among all groups (P＞0.05).④The trypsin activity in the liver of fish in L10 group was significantly higher than that in L16,L19,and L22 groups (P＜0.05).In the intestines of fish,the trypsin activity in L19 and L22 groups was significantly lower than that in L10 group (P＜0.05),and the lipase activity in L22 group was significantly higher than L10 and L13 groups (P＜0.05).⑤In the livers of the experimental fish,the glutathione peroxidase activity in L19 group was significantly higher than L10 group (P＜0.05),and the catalase activity was significantly higher than L10 and L22 groups (P＜0.05).The malondialdehyde content in L22 group was significantly higher than that in all other groups (P＜0.05). 【Conclusion】 An appropriate dietary lipid level could enhance the growth performance,increase body lipid content,and improve the antioxidant capacity of hybrid snakehead larval.Based on the comprehensive evaluation of growth performance and antioxidant capacity,the recommended dietary lipid requirement was 17.71%-19.39%.

Key words: dietary lipid level; hybrid snakehead larvae; biochemical parameters; digestion; antioxidant capacity