Abstract: In recent years,respiratory Coronaviruses have become a focus of research.They mainly infect ciliated cells and goblet cells of the host trachea,resulting in loss of tight connections between cells,damage the integrity of the respiratory system barrier,and then cause low respiratory tract infection.The trachea epithelium cells (TECs),as an important barrier against pathogen invasion in the body,are also the target cells of many pathogens,and are an important model for the study of respiratory Coronaviruses.During the host-virus interactions,respiratory Coronaviruses preferentially attack ciliary cells,resulting in ciliary clearance dysfunction and destruction of the defensive barrier of the tracheal epithelium,thus exacerbating secondary infection.The authors summarized the morphological structure and physiological function of tracheal epithelial cells,and focused on the methods of isolation and culture of tracheal epithelial cells in vitro,as well as their advantages and disadvantages.The mechanism of interaction between respiratory pathogens and their hosts was further discussed，in order to provide a reference for the isolation and culture of tracheal epithelial cells,and provide support for the study of the infection mechanism and prevention strategy of respiratory Coronavirus.

Key words: tracheal epithelial cells; isolation; culture; respiratory Coronaviruses