检测猪圆环病毒2型抗体SPA胶体金免疫层析方法的建立

Abstract

Abstract: Colloidal gold was prepared with the tri-sodium citrate reduction method. To develop gold immunochromatographic assay for detection of specific antibodies of porcine circovirus type 2, protein A labelled colloidal gold was sprayed onto a conjugate pad, meanwhile PCV-2 capid protein and anti-protein A antibodies were striped in the test line position and in the control line position respectively. The color was appeared both in the test line position and in the control line position when the sample was positive. The color was appeared only in the control line postion when the sample was negative. Compared with ELISA, the sensitivity and specificity of the assay were 89.19% and 100% respectively. No cross-reactivity with PCV-1，HCV，PRV，PRRSV，PPV positive serum samples was observed.

Key words: porcine circovirus type 2; gold immunochromatographic assay; antibody detection; staphylococcal protein A

CLC Number:

S852.65⁺9.2

FAN Xiao-juan;LI Gang;SHI Li-jun;ZHANG Jin-xiu;LI Wei. Detection of Specific Antibodies of Porcine Circovirus Type 2 with Gold Immunochromatographic Assay[J]. China Animal Husbandry & Veterinary Medicine, 2009, 36(7): 158-160.

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Detection of Specific Antibodies of Porcine Circovirus Type 2 with Gold Immunochromatographic Assay

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