2株鸡传染性支气管炎病毒的分离鉴定及S1基因的序列分析

Abstract

Abstract: S1 gene of two strains of infectious bronchitis virus (IBV) isolated in Guangxi and Sichuan were amplified by RT-PCR technique and the cDNA sequences of those strains were determined and analyzed. Phylogenetic relations based on the nucleotide and amino acid sequences were analyzed among isolates and the reference strains in GenBank by DNAStar software. The results demonstrated that isolates had close relationship with J2, Q1 and T3, and less relationship with traditional IBV vaccine strains H120 and 4/91.

Key words: infectious bronchitis virus; PCR; sequence analysis

CLC Number:

S852.65⁺9.6

ZHOU Quan;GUAN Feng-xia;GAO Heng;LIU Ling;LI Min;SONG Yong-feng;ZHOU Li;WEN Na-xiang. Isolation and Identification and Sequence Analysis of S1 Gene of Two Strains of Infectious Bronchitis Virus[J]. China Animal Husbandry & Veterinary Medicine, 2010, 37(8): 37-40.

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Isolation and Identification and Sequence Analysis of S1 Gene of Two Strains of Infectious Bronchitis Virus

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