Abstract:

According to the heart fatty acid-binding protein (H-FABP) gene sequence of yak from GenBank, 13 pairs of primers were designed to amplify the whole gene sequence. The H-FABP gene of BLACK cattle was amplified, sequenced and analyzed. The nucleotide sequence was detected and the amino acid sequence of this gene was deduced. Then we compared to deduce amino acid sequence with the homologous H-FABP gene of other species. A phylogenetic tree of the H-FABP gene of BLACK cattle and other species was constructed with MEGA 4.0. The results showed that H-FABP gene of BLACK cattle(FJ756345) had 4 exons (73, 173, 102 and 54 bp) and 3 introns (3463, 1892 and 1494 bp). After comparison, the identities of the nucleotide sequence of BLACK cattle H-FABP gene with that of yak, general cattle, goat, pig, horse, human, rat, mouse and chicken were 99.3%, 99.0%, 96.3%, 92.5%, 89.8%, 88.3%, 83.3%, 82.8%, 75.6%, respectively, and the identities of the amino acid sequence of BLACK cattle H-FABP gene with that of yak, general cattle, goat, pig, horse, hu-man, rat, mouse and chicken were 99.2%, 99.2%, 96.2%, 93.2%, 91.7%, 88.7%, 86.5%, 86.5%, 77.4%, respectively The phylogenetic results showed that these species were classified into 2 branches as a whole, in which the chicken were on one branch and BLACK cattle, yak, general cattle, goat, pig, horse, human, rat and mouse were another branch. The H-FABP gene of BLACK cattle was highly conserved, the phylogenetic tree of the H-FABP gene accords with the order of evolution.

Key words: BLACK cattle; H-FABP gene; sequencing; sequence analysis