FABP3基因多态性及其对绵羊泌乳性状的影响

doi:10.16431/j.cnki.1671-7236.2025.11.023

摘要/Abstract

摘要： 【目的】筛选脂肪酸结合蛋白3(fatty acid binding protein 3,FABP3)基因的单核苷酸多态性(single nucleotide polymorphism,SNP)位点，分析其与绵羊泌乳性状间的关联性，为绵羊泌乳性状分子遗传研究提供基础数据。【方法】以460只泌乳绵羊为研究对象，包括东佛里生羊32只、湖羊56只、东佛里生羊与湖羊杂交1代(F1)197只和东佛里生羊与湖羊杂交2代(F2)175只。采集血样提取DNA，将PCR扩增产物混池后，采用Sanger测序筛选FABP3基因SNP位点，并用五引物扩增受阻突变体系(penta-primer amplification refractory mutation system,PARMS)法对SNP位点进行分型，分析该基因核苷酸序列变异对绵羊泌乳性状的影响。【结果】在绵羊FABP3基因第2、3内含子区域各发现1个SNP，分别是c.246+20 G/A(SNP1)和c.348+12 C/T(SNP2)。分型结果显示，SNP1位点有AA、AG和GG 3种基因型，其中GG为F1和F2群体的优势基因型，AG为湖羊的优势基因型，G为F1和F2群体优势等位基因；SNP2位点有TT、TC和CC 3种基因型，TC为湖羊和F1群体的优势基因型，TT为F2群体的优势基因型，T为F1和F2群体的优势等位基因。相关性分析结果显示，绵羊FABP3基因AG和GG基因型个体的乳脂率显著高于AA基因型(P＜0.05)，等位基因G与较高的乳脂率显著相关(P＜0.05)；TC和TT基因型个体的乳脂率和日泌乳量显著或极显著高于CC基因型(P＜0.05；P＜0.01)，等位基因T与较高的乳脂率显著相关(P＜0.05)。绵羊FABP3基因CA单倍型个体的日泌乳量显著高于TG单倍型个体(P＜0.05)。【结论】本研究在绵羊FABP3基因中发现c.246+20 G/A位点的AG和GG基因型绵羊的乳脂率显著高于AA基因型，而c.348+12 C/T位点的TC和TT基因型绵羊的乳脂率和日泌乳量显著高于CC基因型。试验结果为绵羊泌乳性状分子遗传研究提供了基础数据。

关键词: 绵羊; FABP3基因; 核苷酸变异; 泌乳性状

Abstract: 【Objective】 This study aimed to screen single nucleotide polymorphism (SNP) of fatty acid binding protein 3 (FABP3) gene,and analyze its association with lactation traits in sheep,providing fundamental data for the molecular genetic study of lactation traits. 【Method】 A total of 460 lactating sheep were selected,including 32 East Frisian sheep,56 Hu sheep,197 hybridization F1 of East Frisian×Hu sheep (F1),and 175 hybridization F2 of East Frisian×Hu sheep (F2).Blood samples were collected to extract DNA.After mixing the PCR amplification products in the pool,SNP of FABP3 gene were screened by Sanger sequencing and their genotypes were typed by penta-primer amplification refractory mutation system (PARMS) method.Finally,the effects of nucleotide sequence variation in FABP3 gene on lactation traits of sheep were analyzed. 【Result】 Two SNPs was found in introns 2 and 3 regions of FABP3 gene in sheep,which were c.246+20 G/A (SNP1) and c.348+12 C/T (SNP2),respectively.The genotyping results showed that SNP1 had three genotypes:AA,AG and GG,of which GG was the dominant genotype for F1 and F2 populations,while AG was the dominant genotype for Hu sheep,and G was the dominant allele for F1 and F2 populations.SNP2 had three genotypes:TT,TC and CC,of which TC was the dominant genotype for Hu sheep and F1 population,while TT was the dominant genotype for F2 population,and T was the dominant allele for F1 and F2 populations.Correlation analysis results indicated that the milk fat percentage of AG and GG genotypes of FABP3 gene in sheep was significantly higher than that of AA genotype (P＜0.05),and the allele G was significantly associated with increased milk fat percentage (P＜0.05).The milk fat percentages and average daily milk yields of TC and TT genotypes of FABP3 gene in sheep were significantly or extremely significantly higher than those of CC genotype (P＜0.05 or P＜0.01),and the allele T was significantly linked to increased milk fat content(P＜0.05).Furthermore,the average daily milk yield of CA haplotype of FABP3 gene in sheep was significantly higher than that of TG haplotype (P＜0.05). 【Conclusion】 In this study,the milk fat percentage of AG and GG genotypes at c.246+20 G/A of FABP3 gene in sheep was significantly higher than that of AA genotype,and the milk fat percentage and daily milk production of TC and TT genotypes at c.348+12 C/T in sheep were significantly higher than that of CC genotype.The results provided foundational data for molecular genetic research on lactation traits in sheep.

Key words: sheep; FABP 3 gene; nucleotide variation; lactation traits

中图分类号:

S813.3

郭世伟, 刘圆, 郝志云, 张希云, 车陇杰, 李明娜, 任春燕, 王继卿, 王自科. FABP3基因多态性及其对绵羊泌乳性状的影响[J]. 中国畜牧兽医, 2025, 52(11): 5265-5275.

GUO Shiwei, LIU Yuan, HAO Zhiyun, ZHANG Xiyun, CHE Longjie, LI Mingna, REN Chunyan, WANG Jiqing, WANG Zike. FABP3 Gene Polymorphism and Its Effect on Lactation Traits in Sheep[J]. China Animal Husbandry & Veterinary Medicine, 2025, 52(11): 5265-5275.

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